The perceived need for more rapid and reliable methods for measuring water quality, especially in the area of drinking water safety, has established new interest in the use of the ATP chemiluminescence. In a process environment, or for any process where the microbiological water quality is critical, corrective actions depend on timely data. Traditional plate counting methods take days for results to become available and this may be further increased by laboratory reporting times.
The EZ-ATP® On-line Microbiology Analyzer is not simply a test kit but a unique alternative to current analysis procedures, taking the standard ASTM test method a step further. The luciferin-luciferase reaction is implemented in an on-line, automatic analyzer mainframe. Sampling, analysis and reporting are automatic, which allows fast feedback on the microbial load in the water body.
Overview of features
Complying with internationally accredited ASTM D4012–81 standard test method
Rapid measurement: 10 minutes (including lysis) Complete ATP recovery: detection of intracellular, extracellular and total ATP
Low cost of analysis relative to a large number of results
Smart functions: automatic calibration and cleaning
No bias from the composition of the growth medium such as the case with plate counting
Measuring ranges: 0 – 250 ng/L (0 – 500 pM ATP) for drinking water, or higher
Parameters
The EZ-ATP® is capable of measuring the total ATP portions of any type of bacterial microorganism present in the water sample, such as fecal coliforms (E. coli), sulphate reducing bacteria, nitrifying bacteria, Legionella… A high ATP value indicates a potential risk in surpassing a threshold value of microorganisms in the past or in the near future. The EZ-ATP® allows to differentiate between intracellular and extracellular ATP levels:
Extracellular ATP also called free ATP, or the portion of ATP released by dead cells
Total ATP, obtained after a proprietary, non-chemical lysis of the incoming sample
Intracellular ATP, obtained by calculation (total ATP minus extracellular), or the portion of ATP from the metabolically active living microorganisms
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